Publications

in revision, under review, in preparation

   

Hoedjes KM*, Grath S*, Posnien N*, Ritchie MG*, Schlötterer C* et al. (under review Molecular Ecology) “From whole bodies to single cells: a guide to transcriptomic approaches for ecology and evolutionary biology.”

*equal contribution

 

Medina-Jiménez BI, Budd GE, Pechmann M, Posnien N, Janssen R (under review BMC Evo Devo) “Single-cell sequencing reveals novel insights into spider eye development.”

 

Jackson D#*, Cerveau N*, Posnien N#* (under review, invited review for Frontiers in Zoology) “Assembling and using transcriptomes de novo based on short read data from emerging model organisms; recommendations and cautions for the novice”

*equal contribution; #corresponding authors

 

Torres-Oliva M*, Buchberger E*, Buffry AD*, Kittelmann M, Sumner-Rooney L, Gaspar PMC, Bullinger GC, Guerrero G, Casares F, Arif S#, Posnien N#, Nunes DDS#, McGregor AP#, Almudi I# (in prep) "Differences in orthodenticle expression promote ommatidial size variation between Drosophila species"

preprint available here.

*equal contribution

#corresponding authors  

 

Lu T-H, Wiegleb G, Reim L, Ahrend J, Torres-Oliva M, Buchberger E, Posnien N (in prep) “Genes related to peripodial epithelium development contribute to natural variation in eye size and shape between Drosophila melanogaster and D. mauritiana”

 

Dang TKL*, Reis M*, Posnien N (in prep) „Integration of multi-omics data reveals cellular processes underlying eye size variation between Drosophila americana and D. novamexicana”

*equal contribution

 

Torres-Oliva M*, Buchberger E*, Almudi I, Lu T-H, Posnien N (in prep) “Regulatory divergence in closely related Drosophila species depends on the architecture of developmental gene regulatory networks.”

*equal contribution

 

2023

 

Posnien N, Hunnekuhl VS, Bucher G (2023). “Gene Expression Mapping of the Neuroectoderm across Phyla – Conservation and Divergence of Early Brain Anlagen between Insects and Vertebrates.” eLife 12 (September). 

doi: 10.7554/elife.92242

 

2022

 

Wiegleb G, Reinhardt S, Dahl A, Posnien N (2022). "Tissue dissociation for single-cell and single-nuclei RNA sequencing for low amounts of input material." Frontiers in Zoology 19:27.

doi: 10.1186/s12983-022-00472-x

preprint: here. 

 

Posnien N#, Beldade P, Casares F (2022) "Editorial: Evolution of Postembryonic Development" Front. Ecol. Evol.

doi: 10.3389/fevo.2022.859670

#corresponding author

 

2021

 

Reis M*, Siomava N*, Wimmer EA, Posnien N (2021) “Conserved and Divergent Aspects of Plasticity and Sexual Dimorphism in Wing Size and Shape in Three Diptera." Front. Ecol. Evol. 9:660546.

doi: 10.3389/fevo.2021.660546

preprint: here.  

 

Buchberger E, Bilen A, Ayaz S, Salamanca D, Matas de las Heras C, Niksic A, Almudi I, Torres-Oliva M, Casares F, Posnien N (2021) “Variation in pleiotropic hub gene expression is associated with interspecific differences in head shape and eye size in Drosophila.” Molecular Biology and Evolution; msaa335.

doi: 10.1093/molbev/msaa335

 

2020

 

Reis M, Wiegleb G, Claude J, Lata R, Horchler B, Ha N-T, Reimer C, Vieira CP, Vieira J, Posnien N (2020). “Multiple loci linked to inversions are associated with eye size variation in species of the Drosophila virilis phylad.” Scientific Reports.

doi: 10.1038/s41598-020-69719-z

 

Kapun M, Barrón MG, [… incl. Posnien N…], Flatt T, González J (2020). "Genomic Analysis of European Drosophila melanogaster Populations Reveals Longitudinal Structure, Continent-Wide Selection, and Previously Unknown DNA Viruses." Molecular Biology and Evolution; msaa120.

doi: 10.1093/molbev/msaa120

 

Cordellier M+, Schneider JM+, Uhl G+, Posnien N+ (2020). "Sex differences in spiders: from phenotype to genomics." Development Genes and Evolution.

doi: 10.1007/s00427-020-00657-6

+ equal contribution/corresponding authors

 

Herndon N, Shelton J, [… incl. Posnien N…], Brown SJ, Bucher G (2020). "Enhanced genome assembly and a new official gene set for Tribolium castaneum." BMC Genomics 21:47. 

doi: 10.1186/s12864-019-6394-6

 

2019 

 

Buchberger E, Reis M, Lu T-H, Posnien N (2019) "Context dependent gene regulation and implications for evolutionary studies." Genes 10(7), 492 

doi: 10.3390/genes10070492

 

2018

 

Reis M, Vieira PC, Lata R, Posnien N, Vieira J (2018) "Origin and consequences of chromosomal inversions in the virilis group of Drosophila." Genome Biology and Evolution; evy239,

doi: 10.1093/gbe/evy239

 

Posnien N (2018) “Phenotyping in evo-devo” in Modelling and Quantitative Approaches to Evo-Devo (section editor: Philipp Mitteröcker) as part of Evolutionary Developmental Biology - A Reference Guide (Editors: Laura Nuño de la Rosa and Gerd B. Müller); pp. 1–12. Cham: Springer International Publishing.

doi: 10.1007/978-3-319-33038-9_121-1 

 

Torres-Oliva M, Schneider J, Wiegleb G, Kaufholz F, Posnien N (2018) “Dynamic genome wide expression profiling of Drosophila head development reveals a novel role of Hunchback in retinal glia cell development and blood-brain barrier integrity.” PLoS Genet 14(1):e1007180.

 

2017

 

Pechmann M, Benton MA, Kenny NJ, Posnien N, Roth S (2017) „A novel role for Ets4 in axis specification and cell migration in the spider Parasteatoda tepidariorum.” eLife 2017;6:e27590.

doi: 10.7554/eLife.27590

 

Schwager EE, […], Posnien N*, Richards S*, McGregor AP* (2017) “The house spider genome reveals a whole genome duplication during arachnid evolution.” BMC Biology 201715:62. 

doi: 10.1186/s12915-017-0399-x

 

Khatib AA, Siomava N, Iannini A, Posnien N, Casares F (2017) Specific expression and function of the Six3 optix in Drosophila serially homologous organs. Biology Open 2017

 

2016

 

Torres-Oliva M, Almudí I, McGregor AP, Posnien N (2016) A robust (re-)annotation approach to generate unbiased mapping references for RNA-seq-based analyses of differential expression across closely related species. BMC Genomics.

doi: 10.1186/s12864-016-2646-x

 

Siomava N, Wimmer EA*, Posnien N* (2016) Size relationships of different body parts in the three dipteran species Drosophila melanogaster, Ceratitis capitata and Musca domestica. Dev Genes Evol.

doi: 10.1007/s00427-016-0543-6

 

Prpic N-M, Posnien N (2016) Size and shape—integration of morphometrics, mathematical modelling, developmental and evolutionary biology. Dev Genes Evol 1–4.

doi: 10.1007/s00427-016-0536-5

 

2015

 

Schomburg C, Turetzek N, Schacht MI, Schneider J, Kirfel P, Prpic N-M and Posnien N (2015) Molecular characterization and embryonic origin of the eyes in the common house spider Parasteatoda tepidariorum. EvoDevo 2015, 6:15.

 

press coverage: 

Press release Uni Göttingen

Der Standard

Göttinger Tageblatt

 

2014

 

Hilbrant M, Almudi I, Leite DJ, Kuncheria L, Posnien N, Nunes MDS, McGregor AP (2014) Sexual dimorphism and natural variation within and among species in the Drosophila retinal mosaic. BMC Evolutionary Biology 14: 240.

 

Posnien N*+, Zeng V*, Schwager EE, Pechmann M, Hilbrant M, Keefe JD, Damen, WGM, Prpic, NM, McGregor AP, Extavour CG+ (2014) A Comprehensive Reference Transcriptome Resource for the Common House Spider Parasteatoda tepidariorum. PLoS ONE 9: e104885.

+corresponding authors 

 

press coverage: 

HNA

Göttinger Tageblatt

 

Hogvall M, Schönauer A, Budd GE, McGregor AP, Posnien N, Janssen, R (2014) Analysis of the Wnt gene repertoire in an onychophoran provides new insights into the evolution of segmentation. EvoDevo 5: 14.

 

Janssen R, Posnien N (2014). Identification and embryonic expression of Wnt2, Wnt4, Wnt5 and Wnt9 in the millipede Glomeris marginata (Myriapoda: Diplopoda). Gene Expression Patterns 14: 55–61.

 

2013

 

Arif S, Hilbrant M, Hopfen C, Almudi I, Nunes MDS, Posnien N, Kuncheria L, Tanaka K, Mitteröcker P, Schlötterer C, McGregor AP (2013). Genetic and Developmental Analysis of Differences in Eye and Face Morphology Between Drosophila simulans and Drosophila mauritiana. Evolution & Development 15, 4 (2013): 257–267. 

 

Kittelmann S, Ulrich J, Posnien N, Bucher G (2013) Changes in anterior head patterning underlie the evolution of long germ embryogenesis. Developmental Biology 374: 174–184.

 

2012

 

Fu J*, Posnien N*, Bolognesi R, Fischer TD, Rayl P, Oberhofer G, Kitzmann P, Brown SJ, and Bucher G (2012) Asymmetrically Expressed Axin Required for Anterior Development in Tribolium. PNAS 109(20): 7782–7786

 

Posnien N*, Hopfen C*, Hilbrant M, Ramos-Womack M, Murat S, Schönauer A, Herbert SL, et al. (2012) Evolution of Eye Morphology and Rhodopsin Expression in the Drosophila melanogaster Species Subgroup. PLoS ONE 7(5): e37346

 

2011

 

Posnien N, Koniszewski N, Bucher G (2011) Insect Tc-six4 marks a unit with similarity to vertebrate placodes. Dev Biol 350: 208-216

 

Posnien N*, Koniszewski NDB*, Hein HJ, Bucher G (2011) Candidate Gene Screen in the Red Flour Beetle Tribolium Reveals Six3 as Ancient Regulator of Anterior Median Head and Central Complex Development. PLoS Genet 7(12): e1002416. doi:10.1371/journal.pgen.1002416

 

2010

 

Posnien N, Bucher G (2010) Formation of the insect head involves lateral contribution of the intercalary segment, which depends on Tc-labial function. Dev Biol 338: 107-116.

 

Posnien N, Schinko J, Kittelmann S, Bucher G (2010) Genetics, development and composition of the insect head - A beetle's view. Arthropod Struct Dev 39: 399-410.

 

Steinmetz PRH, Urbach R, Posnien N, Eriksson J, Kostyuchenko RP, Brena C, Guy K, Akam M, Bucher G, Arendt D (2010) Six3 demarcates the anterior-most developing brain region in bilaterian animals. EvoDevo 2010, 1:14

 

2009

 

Posnien N, Bashasab F, & Bucher G (2009) The insect upper lip (labrum) is a nonsegmental appendage-like structure. Evol Dev 11(5):480-488

 

Yang X, Weber M, Zarinkamar N, Posnien N, Friedrich F, et al. (2009) Probing the Drosophila retinal determination gene network in Tribolium (II): The Pax6 genes eyeless and twin of eyeless. Dev Biol 333: 215-227.

 

Posnien N, Schinko J, Grossmann D, Shippy TD, Konopova B, et al. (2009) RNAi in the Red Flour Beetle (Tribolium). CSH Protoc 2009: pdb prot5256.

 

Schinko J, Posnien N, Kittelmann S, Koniszewski N, Bucher G (2009) Single and double whole-mount in situ hybridization in red flour beetle (Tribolium) embryos. CSH Protoc 2009: pdb prot5258.

 

2008

 

Richards S, Gibbs RA, Weinstock GM, Brown SJ, Denell R, et al. (including Posnien N) (2008) The genome of the model beetle and pest Tribolium castaneum. Nature 452: 949-955.

 

Schinko JB, Kreuzer N, Offen N, Posnien N, Wimmer EA, et al. (2008) Divergent functions of orthodenticle, empty spiracles and buttonhead in early head patterning of the beetle Tribolium castaneum (Coleoptera). Dev Biol 317: 600-613.

 

* - equal contribution